منابع مشابه
Storage, Handling, and Distribution of Frozen Equine Semen
With the recent acceptance of frozen semen by the American Quarter Horse Association and the American Paint Horse Association, the overall interest and use of this breeding method has increased significantly in recent years. Proper handling and maintenance of semen frozen and stored in liquid nitrogen is critical to the success of artificial insemination with frozen semen. International distrib...
متن کاملBasic aspects of frozen storage of semen.
Basic concepts of cryopreservation and the causes of cryoinjury are reviewed. The possible roles of cryoprotectants and additives are considered in the context of their putative interactions with the sperm plasma membrane. Modern approaches to the laboratory assessment of spermatozoa after freeze-thawing are also briefly discussed.
متن کاملThe clinical use of frozen semen banks.
In humans, use of frozen semen is still limited to the activities of a few centers. Procedure for freezing semen involves adding .1 ml of glycerol for each milliliter of semen to be frozen. The specimen is then transferred into sterilized ampules in 1 ml quantities and sealed. The ampules are then suspended in liquid nitrogen vapor at a temperature of -99 degrees C for 1/2 hour. Following t...
متن کاملBreeding With Chilled And Frozen Semen
Artificial Insemination (AI) involves the introduction of sperm into the reproductive tract of the mare without natural mating. AI in the horse was first practiced long ago. Ancient Arabian texts describe how mares were successfully inseminated. In the late 18th Century, an Italian scientist took the idea up again. He performed a series of studies in which he placed stallion semen in the snow a...
متن کاملFrench field results (1985-2005) on factors affecting fertility of frozen stallion semen.
Results on procedures for freezing stallion semen and the subsequent fertility during 20 years are presented. The present system applied in French National Stud includes: (1) a freezing protocol (dilution in milk, centrifugation and addition of freezing extender (INRA82+egg yolk (2%, v/v)+glycerol (2.5%, v/v) at 22 degrees C, a moderate cooling rate to 4 degrees C and freezing at -60 degrees C/...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1955
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(55)95130-4